Welcome to the Tribolium Group Göttingen!

Tribolium castaneum the red flour beetle

Synopsis: We work on the developmental genetics of the red flour beetle Tribolium castaneum. Our goal is to identify genes that are responsible for head development, to reveal their interactions and understand the pattering principles. Both epidermis and brain stem cells (the so called neuroblasts) arise from the same tissue and use - at least in part - the same patterning cues. Therefore, we regard brain and epidermis as two outcomes of the same early patterning processes that should be studied together. Finally, we aim to understand how the insect head evolved thereby contributing to the ongoing discussion on the infamous "arthropod head problem"

Head patterning:
We seek to understand the formation of the entire head from early regionalization to morphogenesis. To identify new genes involved in head development, we have carried out an RNAi screen and have identified head mutants from the "GEKU" insertional mutagenesis screen, a collaboration of the Ernst Wimmer lab with three more labs in Germany and the USA. currently, we are investigating the functions of a subset of these.

Neural development:
With respect to brain development, we want to understand the early signals that give neural stem cells (neuroblasts) different identitites. We focus on a subset of neuroblasts that is located in a region with similarity to the anterior portion of the vertebrate forebrain and that contribute to the central complex.

Evolution:
Another aim is to resolve the old and infamous "arthropod head problem": Still there is no agreement on how the insect head evolved and on the number of segments that compose the insect head, the contribution of nonsegmental anterior tissue and the nature of the labrum. Therefore, we interprete our results also with respect to changes that underlie head evolution.

Expanding the tool kit:
We invest in expanding the technical possibilities of our model system. We have established heat-shock mediated misexpression and the UAS/Gal4 misexpression system as well as in vivo imaging using transgenic imaging lines.

iBeetle: Functional genomics of insect development and metamorphosis:
Together with Prof. Martin Klingler I am leading the DFG research unit (Forschergruppe) FOR 1234: iBeetle - Functional genomics of insect development and metamorphosis. We perform a genome-wide RNAi screen in order to detect novel gene functions. Please refer to the iBeetle homepage for more details.

On these pages you will find information about our research, you may get to know the members of the Göttingen Tribolium Group or surf the links to other Tribolium groups. On the protocols site you are invited to download the Tribolium beetle book that lists information and protocols that we are using in the lab. If you have become interested in our work and have further questions please feel free to contact me any time!

Gregor

© Gregor Bucher, last update: January 2012