Analyse der systemischen Wirkung von
Infektionen wurzelbürtiger Pilze auf ausgewählte Brassicaceen
unter
Berücksichtigung von multitrophen Interaktionen mit Insekten
und
mikrobiellen Pathogenen
Influence of Verticillium factors on the
cellbiology of the roots of Arabidopsis
thaliana and Brassica rapus
The
cytoskeleton
of
plants has structural function and provides a network
of transport and signalling within the cell. Upon fungal infection a
profound rearrangement of the actin cytoskeleton takes place. Changes
in the cytoskeleton may be part of the pathogen defence systems of
plants. The aim of this proposal is to investigate the effect of Verticillium dahliae and Verticillium longisporum infection
on the actin cytoskeleton of Arabidopsis
thaliana and Brassica napus
along with the developmental consequences of such effects. The activity
of Verticillium extracts
containing putative toxins will be tested in transgenic plants
expressing fimbrin-GFP to allow visualisation of the actin cytoskeleton
in vivo and real time. Changes in structure and dynamics of the actin
cytoskeleton affect endo- and exocytosis and possibly result in changes
in the localisation of auxin carriers. As a consequence root growth and
development might be disturbed. These processes will be studied using
subcellular markers in plants treated with Verticillium extracts. (click here for the german version)
Abbildung:
Visualisation of the actin-cytoskeleton in root cells of Arabidopsis thaliana using
fimbrin-GFP.