Peer reviewed publications


Farnworth MS, Eckermann K, Bucher G (2020) Sequence heterochrony led to a gain of functionality in an immature stage of the central complex: a fly-beetle insight Plos Biology in press

Herndon N, Shelton J, Gerischer L, ... Stanke* M, Brown* SJ, Bucher* G (2020) Enhanced genome assembly and a new official gene set for Tribolium castaneum BMC Genomics 21,47 (2020);8:e49065

Schacht* MI, Schomburg* C, Bucher G (2020) six3 acts upstream of foxQ2 in labrum and neural development in the spider Parasteatoda tepidariorum Development, Genes and Evolution


He B, Büscher M, Farnworth M.S, Strobl F, Stelzer E, Koniszewski NDB, Mühlen D, Bucher G (2019) An ancestral apical brain region contributes to the central complex under the control of foxQ2 in the beetle Tribolium eLife 2019;8:e49065

Schultheis D, Weißkopf M, ... Bucher G, Frasch M. (2019) A Large Scale Systemic RNAi Screen in the Red Flour Beetle Tribolium castaneum Identifies Novel Genes Involved in Insect Muscle Development. G3 (Bethesda) 2019 Apr 9;9(4):1009-1026. doi: 10.1534/g3.118.200995.


Hu Y, Schmitt-Engel C, Schwirz J, Stroehlein N, Richter T, Majumdar U, Bucher G (2018) A morphological novelty evolved by co-option of a reduced gene regulatory network and gene recruitment in a beetle. Proc R Soc B. 285:20181373.

Ansari* S, Troelenberg*, Dao, Richter, Gregor Bucher* and Martin Klingler* (2018) Double abdomen in a short-germ insect: Zygotic control of axis formation revealed in the beetle Tribolium castaneum. PNAS

Dönitz J., Gerischer L, Hahnke S, Pfeiffer S, Bucher G. (2018) Expanded and updated data and a query pipeline for iBeetle-Base. Nucleic Acids Res. 4;46(D1):D831-D835. doi: 10.1093/nar/gkx984.


Kitzmann P, Weißkopf M, Schacht MI, Bucher G foxQ2 has a key role in anterior head and central brain patterning in insects. Development 2017 : doi: 10.1242/dev.147637

Schwager EE, Sharma PP, Clarke T, Leite DJ, Wierschin T, Pechmann M, et al. The house spider genome reveals an ancient whole-genome duplication during arachnid evolution. BMC Biol. 2017;15:62.

Hahn N, Knorr DY, Liebig J, Wüstefeld L, Peters K, Büscher M, Bucher, G, Ehrenreich H, Heinrich, R The Insect Ortholog of the Human Orphan Cytokine Receptor CRLF3 Is a Neuroprotective Erythropoietin Receptor. Front. Mol. Neurosci. 2017;10


Koniszewski*, NDB, Kollmann* M, Bigham M, Farnworth M, He B, Büscher M, Hütteroth W, Binzer M, Schachtner J, Bucher, G. The insect central complex as model for heterochronic brain development-background, concepts, and tools. Dev. Genes Evol. 2016;226:209-19.


Siemanowski, J., Richter, T., Dao, V. A. & Bucher, G. Notch signaling induces cell proliferation in the labrum in a regulatory network different from the thoracic legs. Dev. Biol. 408, 164-177 (2015).

Ulrich, J., Dao, V.A., Majumdar, U., Schmitt-Engel, C., Schwirz, J., Schultheis, D., Ströhlein, N., Troelenberg, N., Grossmann, D., Richter, T., Dönitz, J., Gerischer, L., Leboulle, G., Vilcinskas, A., Stanke, M., Bucher, G., (2015). Large scale RNAi Screen in Tribolium reveals novel Target Genes for Pest Control and the Proteasome as Prime Target. BMC Genomics.

Schmitt-Engel, C., Schultheis, D., Schwirz, J., Ströhlein, N., Troelenberg, N., Majumdar, U., Dao, V.A., Grossmann, D., Richter, T., Tech, M., Dönitz, J., Gerischer, L., Theis, M., Schild, I., Trauner, J., Koniszewski, N.D.B., Küster, E., Kittelmann, S., Hu, Y., Lehmann, S., Siemanowski, J., Ulrich, J., Panfilio, K.A., Schröder, R., Morgenstern, B., Stanke, M., Buchhholz, F., Frasch, M., Roth, S., Wimmer, E.A., Schoppmeier*, M., Klingler*, M., Bucher*, G., (2015). The iBeetle large-scale RNAi screen reveals gene functions for insect development and physiology. Nat. Commun. 6, 7822. doi:10.1038/ncomms8822 Highligthed in Science Editor´s choice

Dönitz, J., Schmitt-Engel, C., Grossmann, D., Gerischer, L., Tech, M., Schoppmeier, M., Klingler, M. and Bucher, G. (2015). iBeetle-Base: a database for RNAi phenotypes in the red flour beetle Tribolium castaneum. Nucleic Acids Res. gku1054.


Oberhofer, G., Grossmann, D., Siemanowski, J. L., Beissbarth, T. and Bucher, G. (2014). Wnt/b-catenin signaling integrates patterning and metabolism of the insect growth zone. Development dev.112797.


Dönitz J, Grossmann D, Schild I, Schmitt-Engel C, Bradler S, Prpic NM, Bucher G (2013) TrOn: An Anatomical Ontology for the Beetle Tribolium castaneum PlosOne 8:7

Peel A, Grossmann D, Schanda J, Ruge F, Oberhofer G, Schinko JB, Gilles A, Klingler M, Bucher G (2013) Tc-knirps plays different roles in the specification of antennal and mandibular parasegment boundaries and is regulated by a pair-rule gene in the beetle Tribolium castaneum BMC Dev Biol 13:25

Kittelmann S, Ulrich J, Posnien N, Bucher G (2013) Changes in anterior head patterning underlie the evolution of long germ embryogenesis Dev Biol 374(1):174-84

Kitzmann P, Schwirz J, Schmitt-Engel C, Bucher G. (2013) RNAi phenotypes are influenced by the genetic background of the injected strain BMC Genomics 14:5


Schinko JB, Hillebrand K, Bucher G. (2012) Heat shock-mediated misexpression of genes in the beetle Tribolium castaneum. Dev Genes Evol. 2012 Sep7.(5):287-98.

Fu J, Posnien N, Bolognesi R, Fischer TD, Rayl P, Oberhofer G, Kitzmann P, Brown SJ, Bucher G. (2012) Asymmetrically expressed axin required for anterior development in Tribolium. PNAS 109(20):7782-6.


Posnien, N., Koniszewski, N. D. B., Hein, H. and Bucher, G. (2011). Candidate Gene Screen in the red flour beetle Tribolium Reveals six3 as Ancient Regulator of Anterior Median Head and Central Complex Development. Plos Genet 2011

N Posnien, N Koniszewski, G Bucher: Insect Tc-six4 marks a unit with similarity to vertebrate placodes. Dev Biol


Posnien, N. and Bucher, G. (2010). Formation of the insect head involves lateral contribution of the intercalary segment, which depends on Tc-labial function. Dev Biol. 338:107-16.

Schinko, M Weber, I Viktorinova, A Kiupakis, M Averof, M Klingler, EA Wimmer, G Bucher: Functionality of the GAL4/UAS system in Tribolium requires the use of endogenous core promoters. BMC Dev Biol 2010, 10:53.

Steinmetz, R Urbach, N Posnien, J Eriksson, RP Kostyuchenko, C Brena, K Guy, M Akam*, G Bucher*, D Arendt*: Six3 demarcates the anterior-most developing brain region in bilaterian animals. Evodevo 2010, 1:14.


Trauner, J., Schinko, J., Lorenzen, M. D., Shippy, T. D., Wimmer, E. A., Beeman, R. W., Klingler, M., Bucher, G. and Brown, S. J. (2009). Large-scale insertional mutagenesis of a coleopteran stored grain pest, the red flour beetle Tribolium castaneum, identifies embryonic lethal mutations and enhancer traps. BMC Biol 7:73.

Posnien, F Bashasab, G Bucher (2009) The insect upper lip (labrum) is a nonsegmental appendage-like structure. Evol Dev 11:479-487.

Yang X, M Weber, N Zarinkamar, N Posnien, F Friedrich, B Wigand, R Beutel, WG Damen, G Bucher, M Klingler, Friedrich, M (2009): Probing the Drosophila retinal determination gene network in Tribolium (II): The Pax6 genes eyeless and twin of eyeless. Dev Biol 333:215-27.

Kittelmann, M., Schinko, J. B., Winkler, M., Bucher, G., Wimmer, E. A. and Prpic, N. M. (2009). Insertional mutagenesis screening identifies the zinc finger homeodomain 2 (zfh2) gene as a novel factor required for embryonic leg development in Tribolium castaneum. Dev Genes Evol. 2009, 219:399-407.


Tomoyasu, Y., Miller, S.C., Tomita, S. Schoppmeier, M., Grossmann, D., Bucher, G. (2008) Exploring systemic RNA interference in insects: a genome-wide survey for RNAi genes in Tribolium. Genome Biol 9:1, R10

Tribolium Genome Sequencing Consortium# (2008) The first genome sequence of a beetle, Tribolium castaneum, a model for insect development and pest biology. nature 452, 949-55
# mentioned as one of twelve analysis leaders

Cerny, A., Grossmann, D., Bucher*, G. Klingler*, M. (2008) The Tribolium ortholog of knirps and knirps-related is crucial for head segmentation but plays a minor role during abdominal patterning. Dev Biol 321(1):284-94

Schinko, J.B., Kreuzer, N., Offen, N., Posnien, N., Wimmer, E., Bucher, G. (2008) Diverged function of Tribolium orthodenticle, empty-spiracles and buttonhead in head patterning. Dev Biol 317, 600-613

Witek A, Herlyn H, Meyer A, Boell L, Bucher G, Hankeln T. (2008) EST based phylogenomics of Syndermata questions monophyly of Eurotatoria. BMC Evol Biol. 8(1):345.

Boell, L., Bucher, G. (2008) Whole mount in situ hybridization in the Rotifer Brachionus plicatilis representing a basal branch of lophotrochozoans. Dev Genes Evol 8(8):445-51

until 2007

Wohlfrom, H., Klingler, M. and Bucher, G. (2006). knödel is a gene involved in segment maintenance in Tribolium castaneum (Coleoptera). MOD

Cerny*, A. C., Bucher*, G., Schroder, R. and Klingler, M. (2005). Breakdown of abdominal patterning in the Tribolium Kruppel mutant jaws. Development 132, 5353-63.

Bucher, G. and Klingler, M. (2005). Tribolium mae expression suggests roles in terminal and midline patterning and in the specification of mesoderm. Dev Genes Evol 215, 478-481.

Bucher*, G., Farzana*, L., Brown, S. J. and Klingler, M. (2005). Anterior localization of maternal mRNAs in a short germ insect lacking bicoid. Evol Dev 7, 142-9.

Bucher, G. and Klingler, M. (2004). Divergent segmentation mechanism in the short germ insect Tribolium revealed by giant expression and function. Development 131, 1729-40.

Bucher, G., Scholten, J. and Klingler, M. (2002). Parental RNAi in Tribolium (Coleoptera). Curr Biol 12, R85-6.

Berghammer, A., Bucher, G., Maderspacher, F. and Klingler, M. (1999). A system to efficiently maintain embryonic lethal mutations in the flour beetle Tribolium castaneum. Dev Genes Evol 209, 382-9.

Maderspacher*, F., Bucher*, G. and Klingler, M. (1998). Pair-rule and gap gene mutants in the flour beetle Tribolium castaneum. Dev Genes Evol 208, 558-68.

Reviews & Book chapters

Farnworth SM, Eckermann KN, Ahmed HMM, Mühlen DS, He B, Bucher G. (2019) The red flour beetle as model for comparative neural development: Genome editing to mark neural cells in Tribolium brain development. In: Methods in Brain Development. 2nd edition. Humana, New York, NY; 2019.

Hunnekuhl VS, Farnworth MS, Bucher G. Immunohistochemistry and fluorescent whole mount RNA in situ hybridization in larval and adult brains of Tribolium. In: Methods in Brain Development. 2nd edition. Humana, New York, NY; 2019.

Büscher M, Oberhofer G, García-Pérez NC, Bucher G. A protocol for double fluorescent in situ hybridization and immunohistochemistry for the study of embryonic brain development in Tribolium castaneum. In: Methods in Brain Development. Humana, New York, NY; 2019.

G. Bucher, Tribolium (2017), In Reference Module in Life Sciences, Elsevier, ISBN: 978-0-12-809633-8,

G. Bucher, Tribolium (2017), In Reference Module in Life Sciences, Elsevier, ISBN: 978-0-12-809633-8,

Ulrich J., Hu Y., Ansari S., Bucher, G. The red flour beetle - a new genetic model system for pest and vector control with the option of large-scale screening Nova Acta Leopoldina 2016;411:43-55.

Posnien, JB Schinko, S Kittelmann, G Bucher: (2010) Genetics, development and composition of the insect head--a beetle's view. Arthropod Struct Dev 2010, 39:399-410. (review)

Non peer review publications

J Schinko, N Posnien, S Kittelmann, N Koniszewski, G Bucher (2009): Single and Double Whole-Mount In Situ Hybridization in Red Flour Beetle (Tribolium) Embryos. Cold Spring Harbor Protocols 2009,:pdb.prot5258-.

N Posnien, JB Schinko, D Grossmann, TD Shippy, B Konopova, G Bucher (2009) RNAi in the Red Flour Beetle (Tribolium). Cold Spring Harb. Protoc. 2009, doi:10.1101/pdb.prot5256.

SJ Brown, TD Shippy, S Miller, R Bolognesi, RW Beeman, MD Lorenzen, G Bucher, EA Wimmer, M Klingler (2009) The Red Flour Beetle, Tribolium castaneum (Coleoptera): A Model for Studies of Development and Pest Biology. Cold Spring Harbor Protocols 2009, :pdb.emo126-.

Bucher, G. and Wimmer, E. (2005). Beetle a-head. B.I.F. Futura 20, 164-169.

Wigand, B., Bucher, G. and Klingler, M. (1998). A simple whole mount technique for looking at Tribolium embryos. Tribolium Bulletin 38, 281-282.