Publications (selected papers, since 1993)


 
Cytology of prokaryotes, description of new isolates Polyhydroxyalkanoates and other inclusion bodies 
Composition and dynamics of microbial biofilms Structure and function of enzymes in membraneous compartments
Prokaryotic enzyme complexes
Technical contributions  Books 


Cytology of prokaryotes, decription of new isolates

Mayer, F.: Principles of functional and structural organization in the bacteria cell: „Compartments“ and their enzymes. FEMS Microbiol. Rev. 104, 327-346 (1993)

Mayer, F.: Cell Structure. In: Rehm, H.-J., Reed, G. (Hrsg.), Biotechnology, 2nd ed., Vol. 1, pp. 5-46. VCH Verlagsgesellschaft, Weinheim, 1993

Mayer, F.: „Compartments“ in the bacterial cell and their enzymes. ASM News 59, 346-350 (1993)

Hoppert, M., Mayer, F.: Structural aspects of the interaction between phycobilisomes and thylakoid membranes in the unicellular cyanobacterium Synechococcus leopoliensis. Plant Physiol. (Life Sci. Adv.) 14, 11-19 (1995)

Liu, S.Y., Rainey, F.A., Morgan, H.W., Mayer, F., Wiegel, J.: Thermoanaerobacterium aotearoense sp. nov., a slightly acidophilic, anaerobic thermophile isolated from various hot springs in New Zealand, and emendation of the genus Thermoanaerobacterium. Int. J. Syst. Bacteriol. 46 (2), 388-396 (1996)

Engle, M., Li, Y., Rainey, F., Deblois, S., Mai, V., Reichert, A., Mayer, F., Messner, P., Wiegel, J.: Thermobrachium celere, gen. nov., sp. nov., a rapidly growing thermophilic, alkalitolerant, and proteolytic obligate anaerobe. Int. J. Syst. Bacteriol. 46, 1025-1033 (1996)

Gonzalez, J.M., Mayer, F., Moran, M.A., Hodson, R.E., Whitman, W.B.: Microbulbifer hydrolyticus gen. nov., sp. nov., and Marinobacterium georgiense gen. nov., sp. nov., two marine bacteria from a lignin-rich pulp mill waste enrichment community. Int. J. Syst. Bacteriol. 47, 369-376 (1997)

Slobodkin, A., Reysenbach, A.-L., Strutz, N., Dreier, M., Wiegel, J.: Thermoterrabacterium ferrireducens gen. nov., sp. nov., a thermophilic anaerobic dissimilatory Fe(III)-reducing bacterium from a continental hot spring. Int. J. Syst. Bacteriol. 47, 541-547 (1997)

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Composition and dynamics of microbial biofilms

Pohl, W., Hoppert, M., Flies, C., Günzl, B., Ruppert, H., Schneider, J. (2000) Endolithic biofilms: A model for extraterrestrial ecological niches? In: Instruments, Methods and Missions Astrobiology II (Hrsg. R. B. Hoover), Proceedings of SPIE, vol. 3755, pp. 223 - 231. The International Society for Optical Engineering, Bellingham, WA.

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Polyhydroxyalkanoates and other inclusion bodies

Liebergesell, M., Mayer, F., Steinbüchel, A.: Analysis of polyhydroxyalkanoic acid-biosynthesis genes of anoxygenic phototrophic bacteria reveals synthesis of a polyester exhibiting an unusual composition. Appl. Microbiol. Biotechnol. 40, 292-300 (1993)

Pieper-Fürst, U., Madkour, M.H., Mayer, F., Steinbüchel, A.: Purification and characterization of a 14-kDa protein that is bound to the surface of polyhydroxyalkanoic acid granules in Rhodococcus ruber. J. Bacteriol. 176, 4328-4337 (1994)

Liebergesell, M., Sonomoto, K., Madkour, M., Mayer, F., Steinbüchel, A.: Purification and characterization of the polyhydroxyalkanoic acid (PHA) synthase from Chromatium vinosum and localization of the enzyme at the surface of PHA granules. Eur. J. Biochem. 226, 71-80 (1994)

Wieczorek, R., Pries, A., Steinbüchel, A., Mayer, F.: Analysis of a 24-kDa protein associated with the polyhydroxyalkanoic acid granules in Alcaligenes eutrophus. J. Bacteriol. 177, 2425-2435 (1995)

Pieper-Fürst, U., Madkour, M.H., Mayer, F., Steinbüchel, A.: Identification of the region of a 14-kilodalton protein of Rhodococcus ruber that is responsible for the binding of this Phasin to polyhydroxyalkanoic acid granules. J. Bacteriol. 177, 2513-2523 (1995)

Steinbüchel, A., Aerts, K., Babel, W., Föllner, C., Liebergesell, M., Madkour, M.H., Mayer, F., Pieper-Fürst, U., Pries, A., Valentin, H.E., Wieczorek, R.: Considerations on the structure and biochemistry of bacterial polyhydroxyalkanoic acid inclusions. Can. J. Microbiol. 41, Suppl. 1, 94-105 (1995)

Alvarez, H.M., Mayer, F., Fabritius, D., Steinbüchel, A.: Formation of intracytoplasmic lipid inclusions by Rhodococcus opacus strain PD630. Arch. Microbiol. 165, 377-386 (1996)
 

Mayer, F., Hoppert, M.: Determination of the thickness of the boundary layer surrounding bacterial PHA inclusion bodies, and implications for models describing the molecular architecture of this layer. J. Basic Microbiol. 37, 45-52 (1997)

Föllner, C.G., Madkour, M., Mayer, F., Babel, W., Steinbüchel, A.: Analysis of PHA granule-associated proteins GA20 and GA11 in Methylobacterium extorquens and Methylobacterium rhodesianum. J. Basic Microbiol. 37, 11-21 (1997)

Mayer, F., Madkour, M.H., Pieper-Fürst, U., Wieczorek, R., Liebergesell, M., Steinbüchel, A.: Electron microscopic observations on the macromolecular organization of the boundary layer of bacterial PHA inclusion bodies. J. Gen. Appl. Microbiol. 42, 445-455 (1996)

Figures
 

  • Polyhydroxyalkanoic acid (PHA) synthase at the surface of the boundary layer of PHA inclusion bodies from Chromatium vinosum.
  • Ultrastructural organization of the boundary layer of a PHA inclusion body from Pseudomonas oleovorans. Negative staining.
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    Prokaryotic enzyme complexes

    Gerberding, H., Mayer, F.: Interaction and compartmentalization of the components of bacterial enzyme systems involved in cell energetics. Z. Naturforsch. 48c, 535-541 (1993)

    Specka, U., Mayer, F.: Cellular location, activity states, and macromolecular organization of glycoamylase in Clostridium thermosaccharolyticum. Arch. Microbiol. 160, 284-287 (1993)

    Stupperich, E., Juza, A., Hoppert, M., Mayer, F.: Cloning, sequencing and immunological characterization of the corrinoid-containing N5-methyltetrahydromethanopterin: coenzyme M methyltransferase from Methanobacterium thermoautotrophicum. Eur. J. Biochem. 217, 115-121 (1993)

    Konisky, J., Lynn, D., Hoppert, M., Mayer, F., Haney, P. (1994) Identification of the Methanococcus voltae S-layer structural gene. J. Bacteriol. 176, 1790 - 1792.

    Kricke, J., Varma, A., Miller, D., Mayer, F.: Carboxymethyl cellulase from Bacillus sp.: isolation, macromolecular organization, and cellular location. J. Gen. Appl. Microbiol. 40, 53-62 (1994)

    Reidlinger, J., Mayer, F., Müller, V.: The molecular structure of the Na+-translocating F1F0-ATPase of Acetobacterium woodii as revealed by electron microscopy resembles that of H+-translocating ATPases. FEBS Lett. 356, 17-20 (1994)

    Braks, I., Hoppert, M., Roge, S., Mayer, F.: Structural aspects and immunolocalization of the F420-reducing and non-F420-reducing hydrogenases from Methanobacterium thermoautotrophicum Marburg. J. Bacteriol. 176, 7677-7687 (1994)

    Birkenhäger, R., Hoppert, M., Deckers-Hebestreit, G., Mayer, F., Altendorf, K.H.: The F0 complex of theATP synthase of Escherichia coli: Investigation by electron spectroscopic imaging and immunoelectron microscopy. Eur. J. Biochem. 230, 58-67 (1995)

    Hoppert, M., Mayer, F.: Structural aspects of the interaction between phycobilisomes and thylakoid membranes in the unicellular cyanobacterium Synechococcus leopoliensis. Life Sci. Adv. - Plant Physiol. 14, 11-19 (1995)

    Kolmar, H., Hennecke, F., Götze, K., Janzer, B., Vogt, B., Mayer, F., Fritz, H.-J.: Membrane insertion of the bacterial signal transduction protein ToxR and requirements of transcription activation studied by modular replacement of different protein substructures. EMBO J. 14, 3895-3904 (1995)

    Friedebold, J., Mayer, F., Bill, E., Trautwein, A.X., Bowien, B.: Structural and immunological studies on the soluble formate dehydrogenase from Alcaligenes eutrophus. Biol. Chem. Hoppe-Seyler 376, 561-568 (1995)

    Eismann, K., Mlejnek, K., Zipprich, D., Hoppert, M., Gerberding, H., Mayer, F.: Antigenic determinants of the membrane-bound hydrogenase in Alcaligenes eutrophus are exposed toward the periplasm. J. Bacteriol. 177, 6309-6312 (1995)

    Wilms, R., Freiberg, C., Wegerle, E., Meier, I., Mayer, F., Müller, V.: Subunit structure and organization of the genes of the A1A0 ATPase from the archaeon Methanosarcina mazei Gö1. J. Biol. Chem. 271, 18843-18852 (1996)

    Ducki, A., Grundmann, O., Konermann, L., Mayer, F., Hoppert, M. (1998) glucoamylase from thermoanaerobacterium thermosaccharolyticum: sequence studies and analysis of the macromolecular architecture of the enzyme. J Gen. Appl. Microbiol. 98, 327 - 335.

    Krappmann, S., Helmstaedt, K., Gerstberger, T., Eckert, S., Hoffmann, B., Hoppert, M., Schnappauf, G., Braus, G.H.:  The aroC gene of Aspergillus nidulans codes for a monofunctional, allosterically regulated chorismate mutase. J. Biol. Chem. 274, 22275 - 22282 (1999)

    Hofmann, K., Heinz, E.B., Charles, T.C., Hoppert, M., Liebl, W., Streit, W.R.:  Sinorhizobium meliloti strain 1021 bioS and bdhA gene transcriptions are both affected by biotin available in defined medium. FEMS Microbiol. Lett. 182, 41-44, (2000)
     
     

    Figures:

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    Structure and function of enzymes in membraneous compartments

    Hoppert, M., Braks, I., Mayer, F.: Stability and activity of hydrogenases of Methanobacterium thermoautotrophicum and Alcaligenes eutrophus in reversed micellar systems. FEMS Microbiol. Lett. 118, 249-254 (1994)

    Mayer, F., Hoppert, M.: Functional compartmentalization in Bacteria and Archaea: A hypothetical interface between cytoplasmic membrane and cytoplasm. Naturwiss. 83, 36-39 (1996)

    Hoppert, M.,  Mayer, F.: Principles of macromolecular organization and Cell function in Bacteria and Archaea. Cell Biochem. Biophys. 31, 247 - 283, (1999)

    Hoppert, M., Mayer, F.: Prokaryotes. Even without membrane-bounded compartments, prokaryotes display a high degree of subcellular organization. Am. Sci. 87, 518 - 525, (1999) <see also http://www.amsci.org/amsci/articles/99articles/Hoppert.html>
     

    Figure: The cartoon of an enzyme entrapped inside a reversed micelle (A) and bound to a membrane (B) illustrates the similarity between the two situations.
     
     
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    Technical contributions
     

    Hoppert, M., Mayer, F.: Electron microscopic technique for immunocytochemical localization of enzymes in methanogenic archaea. In: Archaea. A laboratory
    manual (Robb, F.T., Place, A.R., Sowers, K.R., Schreier, H.J., DasSarma, S., Fleischmann, E.M., eds.), pp. 269-278, Cold Spring Harbor Laboratory Press,
    1995

    Vogt, B., Berker, R., Mayer, F.: Improved contrast by a simplified post-staining procedure for ultrathin sections of resin-embedded bacterial cells: application of ruthenium red. J. Basic Microbiol. 35, 349-355 (1995)
     
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    Books

    Mayer, F.: Cytology and morphogenesis of bacteria (Volume of Handbook of Plant Anatomy). Gebrüder Bornträger, Berlin, Stuttgart (1986)

    Robinson, D.G., Ehlers, U., Herken, R., Herrmann, B., Mayer, F., Schürmann, F.-W.: Methods of preparation for electron microscopy - an introduction for the biomedical sciences. Springer, Berlin, Heidelberg, New York (1987)

    Mayer, F. (ed.): Methods in Microbiology, Vol. 20: Electron Microscopy in Microbiology. Academic Press, London (1988)

    Hoppert, M, Holzenburg, A. Electron Microscopy in Microbiology. Bios Scientific Publ. Oxford (1998)

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