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New genes controlling blastoderm formation
We have a collection of
mutations in germline clones on the X and II chromosome. These mutations
cause defects in specific aspects in early development.We have finished the
screening and cloned the most promising ones for the X-chromosome. The analysis of some of these
genes has been published (X161/RNPII-215, xit), while others are currently analysed (PP-V, SHMT, kur) or have shown
to be outside of our interests (e. g. wabl,zw-5)
In addition,
we recently performed an EMS mutagenesis experiment together with the laboratory of S Luschnig (Zürich)
and isolated new
mutants in germline clones on the 3L and 3R chromosome arms. Currently, we are characterising
the mutant phenotype by histological analysis and live imaging. Promising mutants are
being mapped and cloned. For cloning we are now using whole-genome sequencing.
(see Grosshans 2005, Brandt 2006,
Sung 2013, Zhang 2014)
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