Karlovsky lab: publications since 2005

Most papers can be downloaded from links provided below under Open Access policy. Reprints not available for download can be ordered at pkarlov at gwdg.de.

2011

• Singh S, Braus-Stromeyer SA, Timpner C, Valerius O, von Tiedemann A, Karlovsky P, Druebert C, Polle A, Braus GH (2011): The plant host Brassica napus induces in the pathogen Verticillium longisporum the expression of functional catalase peroxidase which is required for the late phase of disease. Mol Plant Microbe Interact in press [Epub ahead of print]
• Visentin I, Montis A, Döll K, Alabouvette C, Tamietti G, Karlovsky P, Cardinale F (2011): The transcription of genes in the biosynthetic pathway for fumonisin mycotoxins is epigenetically and differentially regulated in the fungal maize pathogen Fusarium verticillioides. Eukaryotic cell, in press [Epub ahead of print]
• Khorassani R, Hettwer Z, Ratzinger R, Steingrobe S, Karlovsky P, Claassen N (2011): Citramalic acid and salicylic acid in sugar beet root exudates solubilize soil phosphorus. BMC Plant Biology 11:111.
• Karlovsky P (2011): Biological detoxification of the mycotoxin deoxynivalenol and its use in genetically engineered crops and feed additives. Appl Microbiol Biotechnol 91:491-504.
• Nutz S, Döll K, Karlovsky P (2011): Determination of the LOQ in real-time PCR by receiver operating characteristic curve analysis: application to qPCR assays for Fusarium verticillioides and F. proliferatum.
  Analytical and Bioanalytical Chemistry 401: 717-726.
• Splivallo R, Ottonello S, Mello A, Karlovsky P (2011): Truffle Volatiles: from chemical ecology to aroma biosynthesis.
  New Phytologist 189: 688-699.
• Ikunaga Y, Sato I, Grond S, Numaziri N, Yoshida S, Yamaya H, Hiradate S, Hasegawa M, Toshima H, Koitabashi M, Ito M, Karlovsky P, Tsushima S (2011): Nocardioides sp. strain WSN05-2, isolated from a wheat field, degrades deoxynivalenol, producing the novel intermediate 3-epi-deoxynivalenol. Applied Microbiology and Biotechnology, 89: 419-427. Download pdf reprint (337 kb).
• Meyer N, Lind N, Karlovsky P, Zahn M, Friedt W, Ordon F (2011): Development of a real-time PCR method for the identification of wheat genotype carrying different eyespot resistance genes. Plant Breeding 130: 16-24.

2010

• Kurtz B, Karlovsky P, Vidal S (2010): Interaction between western corn rootworm (Coleoptera: Chrysomelidae) larvae and root-infecting Fusarium verticillioides. Environmental Entomology 39: 1532-1538. Download pdf reprint (214 kb)
• Floerl S, Druebert C, Aroud HI, Karlovsky P, Polle A (2010): Disease symptoms and mineral nutrition in Arabidopsis thaliana in response to Verticillium longisporum Vl43 infection. Journal of Plant Pathology 92: 695-702. Download pdf reprint (497 kb)
• Häffner E, Karlovsky P, Diederichsen E (2010): Genetic and environmental control of the Verticillium syndrome in Arabidopsis thaliana. BMC Plant Biology 2010, 10/235. Download pdf reprint (959 kb).
• Alkhedir H, Karlovsky P, Vidal S (2010): Effect of light intensity on colour morph formation and performance of the grain aphid Sitobion avenae F. (Homoptera: Aphididae). Journal of Insect Physiology 56: 1999-2005
• Chatterjee S and Karlovsky P (2010): Removal of the endocrine disrupter butyl benzyl phthalate from the environment. Appl Microbiol Biotechnol 87: 61-73. Download pdf reprint (332 kb).
• Becker R, Hettwer U, Karlovsky P, Deising HB, Wirsel SGR (2010): Adaptation of Fusarium graminearum to Tebuconazole yielded descendants diverging for levels of fitness, fungicide resistance, virulence, and mycotoxin production. Phytopathology 100: 444-453. Download pdf reprint (537 kb).
• Martin F, Kohler A, Murat C, Balestrini R, Coutinho PM, Jaillon O, Montanini B, Morin E, Noel B, Percudani R, Porcel B, Rubini A, Amicucci A, Amselem J, Anthouard V, Arcioni S, Artiguenave F, Aury JM, Ballario P, Bolchi A, Brenna A, Brun A, Buée M, Cantarel B, Chevalier G, Couloux A, Da Silva C, Denoeud F, Duplessis S, Ghignone S, Hilselberger B, Iotti M, Marçais B, Mello A, Miranda M, Pacioni G, Quesneville H, Riccioni C, Ruotolo R, Splivallo R, Stocchi V, Tisserant E, Viscomi AR, Zambonelli A, Zampieri E, Henrissat B, Lebrun MH, Paolocci F, Bonfante P, Ottonello S, Wincker P (2010): Périgord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis. Nature, advance online publication.

• Publicity:	Press info Uni Goettingen (German)
  	Broadcast of Deutschlandradio (MP3, in German)
  	Local newspaper (German)
  	INRA press release (English)

2009

• Jäschke D, Dugassa-Gobena D, Karlovsky P, Vidal S, Ludwig-Müller J (2009): Suppression of clubroot development in Arabidopsis thaliana by the endophytic fungus Acremonium alternatum. Plant Pathology 59: 100-111.
• Sirrenberg A, Splivallo R, Ratzinger A, Pawloswki K, Karlovsky P (2009): Auxin production by symbiotic fungi: Bioassay and HPLC-MS analysis. In: Symbiotic Fungi – Principles and Practice, Vol. 18 of Soil Biology Series (Ed. in Chief A. Varma), Springer, pp. 381-392.
• Adejumo T, Hettwer U, Nutz S, Karlovsky P. (2009): Real-time PCR and agar plating method to predict Fusarium verticillioides and fumonisin B1 content in Nigerian maize. Journal of Plant Protection Research 49: 399-404.
• Visentin I, Tamietti G, Valentino D, Portis E, Karlovsky P, Moretti A, Cardinale F. (2009): The ITS region as a taxonomic discriminator between Fusarium verticillioides and F. proliferatum. Mycological Research 113: 1137-1145.
• Ratzinger A, Riediger N, von Tiedemann A., Karlovsky P (2009): Salicylic acid and salicylic acid glucoside in xylem sap of Brassica napus infected with Verticillium longisporum. Journal of Plant Research 122: 571-579. Download pdf reprint (326 kb).
• Splivallo R, Fischer U, Gobel C, Feussner I, Karlovsky P (2009) Truffles regulate plant root morphogenesis via the production of auxin and ethylene. Plant Physiology 150: 2018-2029. Download pdf reprint (326 kb).
• Sirrenberg A, Nutz S, Ratzinger A, Karlovsky P (2009): Bodenmüdigkeit bei Rosen – neue Forschungsansätze. Gartenpraxis 9: 16-20. Download pdf reprint (2500 kb).
• Eynck C, Koopmann B, Karlovsky P, von Tiedemann A (2009): Internal resistance in winter oilseed rape inhibits systemic spread of the vascular pathogen Verticillium longisporum Phytopathology 99: 802-811. Download pdf reprint 788 kb).
• Weiberg A, Karlovsky P (2009): Components of variance in transcriptomics based on electrophoretic separation of cDNA fragments (cDNA-AFLP). Electrophoresis 2009, 30: 2549–2557. Download pdf reprint (527 kb).

2008

• Award for best poster for Sabine at Mycotoxin-Workshop
• Floerl S, Drübert C, Majcherczyk A, Karlovsky P, Kües U, Polle A (2008): Defence reactions in the apoplastic proteome of oilseed rape (Brassica napus var. napus) attenuate Verticillium longisporum growth but not disease symptoms. BMC Plant Biology 8:129. Download pdf reprint (527 kb).
• Brandfass C, Karlovsky P (2008): Upscaled CTAB-Based DNA extraction and real-time PCR assays for Fusarium culmorum and F. graminearum DNA in plant material with reduced sampling error. Int J Mol Sci 9:2306-2321. Download pdf reprint (363 kb).
• Weiberg A, Pohler D, Morgenstern B, Karlovsky P (2008): Improved coverage of cDNA-AFLP by sequential digestion of immobilized cDNA. BMC Genomics 2008, 9:480. Download pdf reprint (415 kb).
• Meinicke P, Lingner T, Kaever A, Feussner K, Göbel C, Feussner I, Karlovsky P, Morgenstern B. (2008): Metabolite-based clustering and visualization of mass spectrometry data using one-dimensional self-organizing maps. Algorithms Mol Biol. 3:9. Download pdf reprint (18 pages, 400 kb).
• Laurentin H, Ratzinger A, Karlovsky P (2008): Relationship between metabolic and genomic diversity in sesame (Sesamum indicum L.) BMC Genomics 9:250. Download pdf reprint (11 pages, 440 kb).
• Utermark J, Karlovsky P (2008): Genetic transformation of filamentous fungi by Agrobacterium tumefaciens. Nature Protocols Online, DOI: 10.1038/nprot.2008.83.
• Karlovsky P (2008): Secondary Metabolites in Soil Ecology. In: Secondary Metabolites in Soil Ecology, Vol. 14 of Soil Biology Series (Ed. in Chief A. Varma), Springer, pp. 1-19. Download Introduction from Springer website or read excerpts.
• Splivallo R (2008): Biological Significance of Truffle Secondary Metabolites. In: Secondary Metabolites in Soil Ecology, Vol. 14 of Soil Biology Series (Ed. in Chief A. Varma), Springer, pp. 141-165. Free abstract, full text for authorized users only.
• Zuther K, Mayser P, Hettwer H, Wu W, Spiteller P, Kindler BLJ, Karlovsky P, Basse CW, Schirawski J (2008): The tryptophan aminotransferase Tam1 catalyses the single biosynthetic step for tryptophan-dependent pigment synthesis in Ustilago maydis. Mol. Microbiol. 68: 152-172.
• Award for prominent work in medical mycology
• Zahran E, Sauerborn J, Abbasher AA, Ahmed EA, Mohukker RI, Karlovsky P, Mohamed EA, Müller-Stöver D (2008): Pesta and alginate delivery systems of Fusarium spp. for biological control of Striga hermonthica (Del.) Benth. under Sudanese field conditions. Biological Control 44: 160-168.

2007

• Sirrenberg A, Göbel C, Grond S, Czempinski N, Ratzinger A, Karlovsky P, Santos P, Feussner I, Pawlowski K (2007): Piriformospora indica can affect plant growth by auxin production. Physiologia Plantarum 131: 581-589.
• Adejumo TO, Hettwer U, Karlovsky P (2007): Survey of maize from south western Nigeria for zearalenone, fumonisin B1, alpha- and beta- zearalenols and enniatins produced by Fusarium species. Food Additives and Contaminants, 24:993-1000.
  Abstract on PubMed with a link to publisher's website.
• Adejumo TO, Hettwer U, Karlovsky P (2007): Occurrence of Fusarium species and tricothecenes in Nigerian maize. Int J Food Microbiol 116:350-357. Abstract on PubMed with a link to publisher's website.
• Hettwer U, Karlovsky P (2007): Multimethode zur Bestimmung von Fusarientoxinen. LaborPraxis 5:42-44. Download pdf reprint (232 kb).
• Laurentin H, Karlovsky P (2007): AFLP fingerprinting of sesame (Sesamum indicum L.) cultivars: identification, genetic relationship and comparison of AFLP informativeness parameters. Genetic Resources and Crop Evolution 54:1437-1446. Download pdf reprint (252 kb).
• Eynck C, Koopmann B, Grunewaldt G, Karlovsky P, Tiedemann A.v. (2007): Differential interaction of Verticillium longisporum and V. dahliae with Brassica napus detected with molecular and histological techniques. European Journal of Phytopathology 118:259-274. Download pdf reprint (640 kb).
• Utermark J, Karlovsky P (2007): Role of zearalenone lactonase in protection of Gliocladium roseum from fungitoxic effects of the mycotoxin zearalenone. Applied and Environmental Microbiology 73:637-642. Download pdf reprint (325 kb).

2005 - 2006

• Petr Karlovsky (2006): Moderne Diagnosemethoden und Nachweisverfahren. Schriftenreihe der Deutschen Phytomedizinischen Gesellschaft 8:104-118. Download pdf reprint (135 kb).
• Schlüter K, Kropf U, Karlovsky P (2006): Untersuchungen zur systemischen Infektion von Fusarium culmorum an Winterweizen in Schleswig-Holstein. Gesunde Pflanzen 58:107–116. Download pdf reprint (587 kb).
• Utermark J, Karlovsky P (2006): Quantification of green fluorescent protein (GFP) fluorescence using real-time PCR thermocycler. BioTechniques 41:150-154. Download pdf reprint (205 kb).
• Laurentin HE, Karlovsky P (2006): Genetic relationship and diversity in a sesame (Sesamum indicum L.) germplasm collection using amplified fragment length polymorphism (AFLP). BMC Genetics 7:10 (10 pages). Open access, [pdf reprint] (294 kb).
• Brandfass C, Karlovsky P (2006): Simultaneous detection of Fusarium culmorum and F. graminearumin plant material by duplex PCR with melting curve analysis. BMC Microbiology 6:4 (10 pages) Open access, [pdf reprint] (321 kb).
• Venkatesh B, Hettwer U, Koopmann B, Karlovsky P (2005): Conversion of cDNA differential display results (DDRT-PCR) into quantitative transcription profiles. BMC Genomics 6:51 (12 pages)
  Open access, [pdf reprint] (437 kb).

Selected publications by Petr Karlovsky 1986 - 2004

A. Detoxification of mycotoxins

• Völkl A., Vogler B., Schollenberger M. and Karlovsky P (2004): Microbial detoxification of mycotoxin deoxynivalenol. Journal of Basic Microbiology 44: 147-156.
• Karlovsky P., Crane E.H., Gilliam J.T. and Maddox J.R. (2004): Compositions and methods of zearalenone detoxification. United States Patent US 6,812,380, 50 pages.
• Crane E.H., Gilliam J.T., Karlovsky P. and Maddox J.R. (2002): Compositions and methods of zearalenone detoxification. WTO Patent Application No. 02/076205, Canadian Patent No. CA 2441691,
• Karlovsky P (1999): Biological detoxifcation of fungal toxins and its use in plant breeding, feed and food production - a review. Natural Toxins 7:1-23.
• Völkl A., Karlovsky P. (1999): Hefen und Tonminerale binden Mycotoxine: Wirksamkeit mineralischer und organischer Substanzen unterschiedlich. Agrarzeitung Ernährungsdienst, 24.04.1999.

B. Molecular genetics of Phytophthora parasitica

• Karlovsky P., Fartmann B., Mühlbauer E. and Prell H.H (1998): ARS elements from mitochondrial DNA of Phytophthora parasitica: functional and structural characterization. Mycological Research 102: 1133-1141.
• de Cock A.W.A.M., Karlovsky P., Jahnke K.-D. and Bahnweg G. (1996): Similarities in restriction fragment patterns of mitochondrial DNAs of Phytophthora species strongly depend on the restriction enzyme used due to heterogeneous base distribution and sequence conservation. Fungal Genetics and Biology 20: 36-42.
• Karlovsky P. (1994): Inhibition of imidazoleglycerolphosphate dehydratase of Phytophthora parasitica by aminotriazole in situ and after cloning and expression of the respective gene (HIS3) in Escherichia coli. J. Phytopath. 141:121-126. [pdf reprint 550 kb]
• Karlovsky P. and Fartmann B. (1992): Genetic code and phylogenetic origin of oomycetous mitochondria. Journal of Molecular Evolution 34:254-258. [pdf reprint 570 kb]
• Prell H.H., Karlovsky P. and Bahnweg G. (1991): Towards transformation in Phytophthora. In: Phytophthora (J.A. Lucas, R.C. Shattock, D.S. Shaw, L.R.Cooke, eds.), Cambridge University Press, Cambridge/New York/Port Chester/Melbourne/Sydney, pp.312-325.
• Karlovsky P. and Prell H.H. (1991): The TRP1 gene of Phytophthora parasitica encoding indole-3-glycerol-phosphate synthase--N-(5'-phosphoribosyl)anthranilate isomerase: structure and evolutionary distance from homologous fungal genes. Gene 109:161-165. [pdf reprint 570 kb]
• Rump A. and Karlovsky P. (1991): Tandem arrangement of tRNAAsp genes in Phytophthora ssp. Gene 102:51-56 [pdf reprint 300 kb]

C. Theory of enzyme kinetics; logic and intuition

• Karlovsky P. (1988): Calculation of individual cleavage rates from partial digests in restriction endonuclease kinetics. J. Theor. Biol. 132: 7-14.
• Karlovsky P. (1988): Mutual competitive inhibition between target sites during the restriction endonuclease digestion of DNA. J. Theor. Biol. 132: 1-6.
• Karlovsky P. (1986): Liar puzzles and logical intuition I. Roz. Mat. Fyz. 65: 221-223.
• Karlovsky P. (1986): Liar puzzles and logical intuition II. Roz. Mat. Fyz. 65: 265-267.
• Karlovsky P. (1986): Re-evaluation of a method calculating cleavage rates at different sites of DNA from partial digestion of end-labelled molecules. Biochem. Biophys. Res. Commun. 138: 778-782.
• Karlovsky P. (1986): Kinetics of circular DNA molecule digestion by restriction endonuclease. Computation of kinetic constants from the time dependence of fragment concentrations. Acta Biotheoretica 35: 279-292.
• Karlovsky P. (1986): A re-evaluation of evidence attributing the difference in cleavage rates of restriction endonuclease at different sites in the substrate to differences in Km values. Biochem. J. 235: 611-612. [pdf reprint 242 kb]

D. Other topics

• Reineke A. and Karlovsky P. (2000): Simplified AFLP protocol: replacement of primer labeling by the incorporation of alpha-labeled nucleotides during PCR. BioTechniques 28: 622-623. BioTechniques 28: 622-623. [pdf reprint 165 kb]
• Reineke A., Karlovsky P. and Zebitz C.P.W. (1999): Suppression of randomly primed PCR products (RAPD) in heterozygous diploids. Molecular Ecology 8: 1449-1455. Molecular Ecology 8: 1449-1455. [pdf reprint 508 kb]
• Reineke A., Karlovsky P. and Zebitz C.P.W. (1999): Amplified fragment length polymorphism analysis of different geographic populations of the gypsy moth, Lymantria dispar (Lepidoptera: Lymantriidae). Bulletin of Entomological Research 89: 79-88.
• Reineke A., Karlovsky P. and Zebitz C.P.W. (1998): Preparation and Purification of DNA from Insects for AFLP Analysis. Insect Molecular Biology 7: 95-99.
• Oppermann B., Karlovsky P. and Reißer W. (1997): M13 DNA fingerprinting in unicellular and filamentous green algae. European Journal of Phycology 32: 103-110.
• Möller E.M., Schilling A.G. and Karlovsky P. (1994): Stripping of hybridization membranes. BioTechniques 17: 682-683.
• Karlovsky P. (1994): Indirect costs of pesticide use. Science 263: 1208.
• Karlovsky P. (1993): Screening for recombinant plasmids using MacConkey agar. Trends in Genetics 9: 375-376. [pdf reprint 109 kb]
• Karlovsky, P. and Wolf, G. (1993): Screening of expression cDNA libraries for polysaccharide hydrolysing enzymes: isolation of cDNA clones encoding cellulase, xylanase and ß-1,3-glucanase of Phytophthora parasitica. Methods in Molecular and Cellular Biology 4: 40-45. [pdf reprint 500 kb]
• Karlovsky P. (1993): Alternatives to X-gal in screening recombinant clones based on pUC-derived plasmid vectors. Methods Enzymol. 217: 335-339. [pdf reprint 412 kb]
• Jelen F., Karlovsky P., Makaturova E., Pecinka P. and Palecek E. (1991): Osmium tetroxide reactivity of DNA bases in nucleotide sequencing and probing of DNA structure. Gen. Physiol. Biophys. 10: 461-473.
• Karlovsky P. and de Cock A.W.A.M. (1991): Buoyant density of DNA-Hoechst 33258 (bisbenzimide) complexes in CsCl gradients: Hoechst 33258 binds to single AT base-pairs. Analytical Biochemistry 194:192-197. [pdf reprint 630 kb]
• Karlovsky P. (1990): Misuse of PCR. Trends Biochem. Sci. 15:419 [pdf reprint 120 kb]
• Karlovsky P., Pecinka P., Vojtiskova M., Makaturova E. and Palecek E. (1990): Protonated triplex DNA in E. coli cells as detected by chemical probing. FEBS Letters 274: 39-42.
• Karlovsky P. (1989): Reprints still in demand. Nature 338: 290.
• Palecek E., Boublikova P. and Karlovsky P. (1987): Osmium Tetroxide recognizes structural distortions at junctions between right- and left-handed DNA in a bacterial cell. Gen. Physiol. Biophys. 6: 593-608.
• Karlovsky P. and Prell H. H. (1987): Screening of pUC plasmid clones for inserts based on growth rate. Nucleic Acids Res. 15: 6753. [pdf reprint 104 kb]
• Karlovsky P. and Vaskova M. (1987): Tn1000 insertional mutagenesis of cloned repressor gene of the phage L: Plasmid oligomerization in the presence of F'lac. Folia Microbiol. 32: 185-194.
• Karlovska L., Brzobohaty B., Karlovsky P., Reich J., Koukalova B. and Bezdek, M. (1987): Transfer of Drosophila melanogaster transposable genetic element mdg-4 into plant cells. Folia Biologica 33: 40-49.
• Karlovsky P. and Kuhrova V. (1986): Specific binding affinity for DNA of the L phage (Salmonella typhimurium) in Escherichia coli. Molec. Biol. Rep. 11: 43-46.
• Karlovsky P. and Vaskova M. (1986): Hot spot for Tn1000 insertions in cloned repressor gene of the L phage. Plasmid 16: 219-221.
• Spanova A. and Karlovsky P. (1986): Integration of transposon Tn10 into phage L (Salmonella typhimurium). Folia Microbiol. 31: 353-363.
• Karlovsky P. and Dadak, V. (1982): Tetraphenylborate-sensitive electrode for measuring membrane potential. Folia Microbiol. 27: 460-464. [pdf reprint 349 kb]